FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33262950
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA47400.033.0892950
GCCGTAT55650.028.43501748
CGCCGTA57550.027.61721447
GCGTCGT59450.026.435412
GATCGGA72050.022.6399382
CCGTATC72100.022.18552849
AGAGCGT79300.020.1663069
AGCGTCG80850.019.73741511
GTCGTGT80600.019.71316514
CGTGTAG80150.019.6082216
TCGTGTA80500.019.56569515
GTAGATC81900.018.97765432
GTATCAT85150.018.86486451
AGATCGG85500.018.6315521
CGTCGTG90350.018.2738513
CATACGA21500.017.67179115
TGGTCGC91050.017.56961843
GGTCGCC94050.017.33910444
TCGCCGT94200.017.20169346
AAGAGCG100550.016.2480348
Produced by FastQC (version 0.11.5)