FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42069527
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG59050.012.26910214
CGCGGGT23650.011.6938591
TCGTATG63400.011.04089146
CCGGGAT73250.010.9443451
CGCAGAC103850.010.6859891
CGTCCCG62150.010.64347736
TATGCCG73250.010.3207563
TAAGTCG37450.09.90642420
CGTATCG36400.09.9037873
CGCTCGT74350.09.83847115
CGTATGC81000.09.7243241
CTCGTAT70450.09.68760645
CGCGGAG51150.09.3764161
CCGGAAT68700.09.3251611
CCGGCAT60550.09.2505551
CCCGTAT52700.09.1006391
CGACCGT20450.09.0728661
TTACACG47950.09.05098632
CGACCTA25550.08.9039924
CTCGTTT67250.08.9015521
Produced by FastQC (version 0.11.5)