FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24448242
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG38700.033.1600643
TATGCCG42450.030.9686446
CGTATGC44450.029.4187744
ACACGTC51400.026.1756913
GATCGGA45050.026.1196461
CTCGTAT50650.025.20070642
ACGTCTG61000.022.33896615
ATCGGAA55650.021.3890042
CACGTCT68200.020.23355914
TCTCGTA66550.018.55775341
ATGCCGT72650.018.33270347
ATCTCGT69250.018.23254640
CGTCTGA87950.015.9636816
CACACGT87650.015.90106112
GCACACG94250.015.73923911
TCGGAAG76900.015.6579793
GTATGCC95000.014.09219145
CCGTCTT93850.013.93384350
GCCGTCT94700.013.66317749
TTACACG29350.013.28148332
Produced by FastQC (version 0.11.5)