FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24448242
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT57800.011.7616911
TATCGCG6351.8189894E-1211.40728520
CGAAGAT83700.010.9257641
TTACACG32350.010.8783532
CTCGGGT56400.010.8299271
TAAGTCG30300.010.81477820
CTCGGTT31150.010.6351731
CGCGTAT4251.1027623E-610.555671
GTATCGA23050.010.3303674
CGTATCG16750.010.3012973
TCGGATT33250.010.274922
CTCGTTT35500.010.1096551
CGATAAG33550.09.97230217
TCGCTCG36700.09.86861814
CCCGTAT25700.09.8021451
CGCGAAA9600.09.70283243
TCGGGTC49800.09.4934962
CTCGTAT27050.09.4405181
CTGGAAT190050.09.1152221
TACACGA42300.09.05388333
Produced by FastQC (version 0.11.5)