FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39545739
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG55350.013.525187514
CGCTCGT69800.010.92290215
CGCAGAC102100.010.8352531
CGAAGAT122600.010.5743961
CCCGATA77700.09.63481169
ACCCGAT79350.09.56522268
CGGAAAT71200.09.3225781
CTCGGAT92650.09.2165071
CGCAAAT49700.09.1818951
ACTCGTG81950.09.17691422
CGCGTAT8303.6379788E-119.1634581
CCGGGAT75450.09.118191
TACTCGT81500.09.10059921
ACTTCGC84700.08.9606711
TCGTATG37050.08.93865545
CTCGTAC83650.08.86680517
TCGTGCG85200.08.74587524
CTCGGGT84900.08.7140671
TTACACG38850.08.70210332
CGCCAAT68450.08.6869991
Produced by FastQC (version 0.11.5)