FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37876300
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG59600.013.71594314
CGCTCGT66850.012.69874915
CGCAGAC101850.011.2379931
CGAAGAT128600.010.6411541
ACTCGTG82050.010.049952522
TACTCGT78900.010.0083421
CCCGATA84850.09.88270169
CTCGGGT85500.09.8396651
CGCGAGT16650.09.758631
TCGGGTC65300.09.6211412
CGCGAAT15200.09.5018221
TTACACG45850.09.29684532
CTCGTAC86450.09.29594217
CCGGGAT75800.09.2887211
TCGTGCG88350.09.21435724
GCTCGTA94000.09.10640716
CTCGGAT88650.09.0420061
CCGATAT91000.09.02272270
CGCTTCG93150.09.00197534
CGTGCGC92500.08.98985425
Produced by FastQC (version 0.11.5)