FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24617731
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26450.029.22529443
CGTATGC28100.027.26577244
TATGCCG28650.026.74239746
ACACGTC36900.023.26817113
GATCGGA37300.022.863841
AGTCACG38850.022.0158928
CTCGTAT40450.020.04029742
ACGGCTA48850.016.9505932
CACGTCT53300.016.62216214
TATCGCG13250.015.74623922
TCTCGTA48650.015.32667641
ATGCCGT52500.015.24518247
TCACGGC57100.014.98080930
GTCACGG60150.014.96065429
CGCGTAA16350.014.8730231
ATCGGAA56900.014.8505592
ACGTCTG59100.014.64360315
CGTATCG18500.014.606543
CACACGT62500.013.90172212
TCGGAAG63500.013.5204753
Produced by FastQC (version 0.11.5)