FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49075551
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG61900.014.56101614
TCGTATG56900.013.68835543
TATGCCG58500.013.31397246
CGCTCGT70700.012.89290915
CGTATGC63800.012.15464244
CGCAGAC102000.011.7985291
CGGGGAT83350.011.0504141
CTCGTAT73800.010.87636342
CCGGGAT90900.010.3715611
TCGTGCG88300.010.20735524
TCGCGCG7003.3673132E-89.9279992
CGTATCG28650.09.8267093
CTCGTAC95650.09.67194417
CGCCTCG95100.09.62057729
ACTCGTG94750.09.51250122
GCTCGTA98550.09.45647916
CGAAGAT144600.09.3742081
CGCGCAT11600.09.3633291
CGCAACC103850.09.23571750
AATCTCG86050.09.16991139
Produced by FastQC (version 0.11.5)