FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49075551
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG70200.014.4008814
CGCTCGT77750.012.91365915
CGCAGAC108900.012.0046851
CGCGTAT7800.011.497891
GCTCGTA103950.010.5882216
TCGTGCG96500.010.57855624
ACTCGTG101900.010.22081322
TACTCGT102100.010.13326821
CTCGTAC104450.09.94293917
CCGGGAT96450.09.6918461
CGCCTCG108950.09.62719929
CGTGCGC107600.09.58370225
CCCGATA109950.09.16516869
CGAAGAT155300.09.1287181
CGCAACC115250.09.03983150
CGCGCAT13450.08.9760481
CTCGGGT92100.08.8762221
TTCGCTC122500.08.78773613
GTACTCG118950.08.755939520
CGAATCG35550.08.734364552
Produced by FastQC (version 0.11.5)