FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15402442
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA409410.2658084997171228TruSeq Adapter, Index 3 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA188420.122331251109402TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG394300.049.4897732
CACTTAG394400.049.2010431
TCACTTA406300.047.48198330
CTTAGGC413250.047.2301333
TCGTATG435350.044.59843443
GTCACTT436100.044.41316629
TATGCCG440400.044.2879746
CTCGTAT432350.044.18572642
CGTATGC442350.044.05451644
ACACGTC450550.044.0097113
ACGTCTG455150.043.6769215
GATCGGA458500.043.4219861
AGTCACT446700.043.40458328
TCTCGTA430500.043.3417641
CACGTCT459800.043.19830714
CAGTCAC453000.043.1603427
CACACGT463750.042.8375412
ATGCCGT458100.042.58418347
ATCGGAA468350.042.4975362
CCGTCTT455900.042.49080750
Produced by FastQC (version 0.11.5)