FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15402442
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA120350.055.95389612
CATACGA111350.055.89776615
GCATACG121650.055.34069414
ATACGAG105950.052.5774116
ACGGCAT150900.049.97581511
AGACGGC173550.049.2289359
AAGACGG176000.049.043298
GCCTAAG108350.048.0207425
TGCCTAA104100.047.85276424
CCTAAGT83450.046.32531426
GACGGCA158850.046.01522410
TACGAGA88950.045.29527717
GAAGACG193150.044.9713177
CGGCGTG57900.044.166412
CTAAGTG84650.043.32354727
GGCATAC158000.042.38679513
ACGAGAT92350.041.68317418
ACGGCGT33000.041.1181111
ACGCGTG13150.040.2545546
AGATCGG144600.039.1893961
Produced by FastQC (version 0.11.5)