FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31050195
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG40150.015.16725514
CGCTCGT50150.012.56159715
CCGGGAT67200.011.6246841
CGAAGAT109750.010.8841731
CGCAGAC76400.010.6833681
CTCGGTT41100.010.4835621
CGCGTTC24050.010.18652914
CGGGGAT64750.010.00869751
CTCGGAT79300.09.8509311
CGTTCGC23550.09.80835816
CGCGGAT24650.09.8056811
TCGCGCG6806.2573235E-109.7786336
TTACACG37000.09.74406932
CGCGGGT22300.09.7394051
CTCGGGT70550.09.7320571
CGCAACC63500.09.70051650
ACCCGAT61950.09.60445268
CGCGAAT13500.09.6009361
CGGAAAT57600.09.4874111
ACTCGTG65850.09.4615622
Produced by FastQC (version 0.11.5)