FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31050195
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG46800.012.91552414
CTCGGGT78400.011.858381
TCGGGTC63500.011.6332712
CGCTCGT54600.011.13444115
CGAAGAT107350.010.9430221
CGCAGAC82950.010.8603881
CGACGAT27000.010.810431
CGCGTTC24900.010.80446214
CGTTCGC24600.010.51081416
CTCGGAT80050.010.1734641
CCGCGTT27450.09.92805213
CGCGAAA15650.09.6008143
CCGGGAT65600.09.5580021
CGGAGAT80650.09.4722521
CGCGAAT13000.09.4244781
CGCGGAT26050.09.4063881
CGCCAAT65800.09.3646581
CGACCGT15200.09.2457631
CGCGTAT6703.11411E-89.1431491
CGGGAAT57050.09.0955411
Produced by FastQC (version 0.11.5)