FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47805136
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46000.017.62344645
CGTATGC51800.016.1829246
TATGCCG53250.015.3535648
TTACGCG24100.013.31229954
CTCGTAT61800.012.50375244
ACACGTC76950.011.70078513
GCGGTAT61650.010.747581
TACGCGA30100.010.65867355
GATCGGA71600.09.7842051
CGCGAAT22000.09.4117651
CGACCTA25700.09.2637474
TCGACCG46600.09.25356331
GCGGGTT47300.09.1928871
TCGCTCG81500.09.10047214
CCCGATA83350.09.02474369
CGCGGAT43750.08.992271
CACGTCT103500.08.93259214
CCGGGAT158400.08.9324631
TTACACG47950.08.92125932
TCTCGTA91400.08.9073343
Produced by FastQC (version 0.11.5)