FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24433702
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA365010.14938792328726935TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG265550.043.9484143
TATGCCG268200.043.79136346
CGTATGC272150.043.2253844
CTCGTAT265800.042.28473342
GATCGGA289650.042.1162641
ACACGTC289750.041.7257913
ACGTCTG291400.041.5203715
ATCGGAA300750.040.619732
CACGTCT299450.040.42026514
TCTCGTA271650.040.39628641
ATGCCGT291500.040.3265747
CACACGT308600.039.26652512
GCACACG314150.038.81444511
TCGGAAG316500.038.6092763
ATCTCGT285550.038.22449540
TGCCGTC308500.038.17146348
GCCGTCT312650.037.57650449
CGTCTGA320700.037.5520716
GATCTCG298650.037.03296339
GTATGCC319400.036.83119645
Produced by FastQC (version 0.11.5)