FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23859048
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG81650.029.35359843
TATGCCG85000.028.62642746
CGTATGC85400.028.18579744
CTCGTAT88550.026.6379242
GTCACGA95400.025.98878329
GATCGGA95400.025.9228941
AGTCACG100500.024.80646928
ACGATCA103650.024.38454232
ACACGTC104450.023.76673113
ATCGGAA104400.023.6860732
CACGATC106250.023.43223431
ACGTCTG110150.022.81856715
TCACGAT110950.022.50177230
TCTCGTA103450.022.26787441
CGATCAG114650.022.01491233
CACGTCT113850.021.89528714
ATGCCGT114450.021.50160247
ATCTCGT108250.021.21280540
TCGGAAG126650.019.5247573
CGTCTGA130050.019.45947316
Produced by FastQC (version 0.11.5)