FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26963709
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG38600.014.71626114
CGCAGAC64350.012.399031
CGCTCGT47500.012.10209715
CGAAGAT92600.012.0816761
TTACACG33450.011.99918632
CGTATCG18150.011.2926613
CGTTCGC16000.010.84220716
CGGGGAT53050.010.4626831
CCCGTAT29200.010.3952191
CTCGTAC58950.010.3861317
CGCAAAT39550.09.9773071
GTATCGA26200.09.6628854
TACTCGT59250.09.64458721
CTCGGAT63000.09.6362041
ACTCGTG59950.09.53197322
CCCGATA58900.09.52864969
GCTCGTA65350.09.42107616
CGCCTCG64250.09.37050129
CGAGAAT52000.09.33970451
CGCGTTC18600.09.32664814
Produced by FastQC (version 0.11.5)