FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38022139
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG44900.013.95173914
CGCTCGT53150.011.91782415
CGACGAT30600.011.1096711
TCGTATG74350.010.92055345
TATGCCG76000.010.8233948
CGCGGGT30700.010.6168441
CGAAGAT121800.010.5601411
CTCGGTT52750.010.2317311
CGTATGC79050.010.22779546
CGCAGAC100700.010.1973911
CCGGGAT76300.010.1052951
CGTATCG25150.09.7405233
TACTCGT68650.09.63661721
CTCGGAT91200.09.6071831
CCCGTAT39350.09.5299341
ACTCGTG68850.09.50700722
CTCGTTT63450.09.2795841
CGCAAAT48300.08.9975741
TCGGGTC59650.08.976422
TCGTGCG70700.08.96003924
Produced by FastQC (version 0.11.5)