FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38022139
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG47500.014.07368514
CGCTCGT56600.011.74911715
CGTATCA44500.011.71910150
CGCAGAC109050.011.297571
CGCGGGT31600.010.965191
CTCGGGT89050.010.65131951
CTCGGAT87350.010.6182031
CGAAGAT122050.010.5243751
CGCGTAT7503.6379788E-1210.2666671
CGCCGTA49100.010.122247
GCCGTAT48300.010.07246448
TCGGGTC64700.010.0618242
TACTCGT71150.09.641602521
ACTCGTG75000.09.56666722
CCGGGAT75850.09.5056031
CGCCAAT63150.09.422011
CGCCGAT56950.09.4029851
CTCGTAC75700.09.38573317
CGACCGT18750.09.3333331
CGCGGAT34450.09.2452831
Produced by FastQC (version 0.11.5)