FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32298325
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACC355370.11002737758072592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA84700.025.2477627
AAGCGAC89150.024.1810220
ATCGAAG105450.023.454396
CCGACAT96950.023.4204771
CGCTATG94450.022.4588629
CCGGTAT121300.022.2181511
TCGCTAT98200.021.70658928
GAACGCT99150.021.6030735
CGACATC103050.021.3881972
AAAAGCG101450.021.28326218
CGAAGGA106900.020.977558
CGGTATT132600.020.0294252
ACGCTTG109850.019.5617337
GACATCG120450.019.4442713
TCGAAGG129000.019.386377
TTGGCCG112900.019.21651541
TGCGCGA65250.019.063969
ACATCGA122200.018.7989544
AGTTTAC148450.017.70478622
AGCCTTA149750.017.69751410
Produced by FastQC (version 0.11.5)