FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32298325
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACC325690.10083804655504582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAC90450.025.31836520
ATCGAAG102600.025.1438086
GTCGCTA91350.025.10667627
CCGACAT101150.024.7325041
CGCTATG95650.023.90587829
CGACATC102550.023.5081082
GAACGCT100350.022.92376335
CCGGTAT112500.022.8831651
TCGCTAT99550.022.865428
AAAAGCG102350.022.6442318
CGAAGGA106650.022.2486868
TCGAAGG121050.021.4254467
CGGTATT120500.021.4087182
GACATCG115600.021.0332983
ACATCGA117850.020.3683454
ACGCTTG113950.020.15753437
TTGGCCG115450.019.92547841
TGCGCGA62450.019.0530059
TATGAAC128750.018.16178732
AGCCTTA140750.018.01008410
Produced by FastQC (version 0.11.5)