EGAF00004859259 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00004859259
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20222839
Sequences flagged as poor quality	0
Sequence length	75
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	68827	0.34034291624435126	TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	20438	0.10106395051654221	TruSeq Adapter, Index 20 (97% over 44bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	21140	53.3031	1
TCGTATG	24845	43.79678	46
TATGCCG	25010	43.603157	49
CGTATGC	25270	43.26235	47
GCACACG	26085	43.184155	12
TATCTCG	25045	42.821182	42
CTCGTAT	25280	42.692883	45
ACACGTC	26515	42.261475	14
AGTCACG	26130	42.102596	29
GATCGGA	26925	41.891003	2
ACGTCTG	27165	41.31069	16
GTCACGT	26370	41.151093	30
TCTCGTA	26365	40.522575	44
CACACGT	27750	40.49111	13
CACGTCT	27790	40.37135	15
GCCGTCT	26980	40.318897	52
ATGCCGT	27020	40.246803	50
ATCGGAA	27970	40.204334	3
ATCTCGT	26665	40.01558	43
TCACGTG	27535	39.54607	31