FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20222839
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA180050.057.90674650
GCGTCGT190500.057.00251412
GTCGCCG186050.056.70750845
GCCGTAT185150.056.6012748
CGCCGTA185300.056.5378447
TGGTCGC185200.054.8730543
AGATCGG206250.054.247211
TCGCCGT185800.053.86606646
CCGTATC194900.053.72220649
AGCGTCG205000.053.71431711
GGTCGCC199900.052.69904744
CGTCGTG206600.052.64504213
TCTCGGT205100.052.08676537
GATCGGA217250.051.8965532
AGAGCGT210500.051.7846379
CGTGTAG212950.050.69949716
GTCGTGT210950.050.68665314
TCGGTGG213200.050.4633139
CGGTGGT202750.050.44443540
TCGTGTA212300.050.36409415
Produced by FastQC (version 0.11.5)