FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28580317
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACG33200.011.53431732
TCGCTCG37150.010.772314
CGCGTAT4905.545371E-810.5756571
CGAAGAT91750.010.5053451
CCGGGAT65350.010.3615021
TATCGCG7151.8189894E-1110.13265522
CGAGAAT55900.09.8264681
CGCCAAT53000.09.4515771
CCGACAT44150.09.076941
CGGGGTT76600.08.9750411
CTCGGAT64650.08.8705751
GTATCGA26200.08.8236474
CGCAAAT35000.08.7848451
CTGGAAT214100.08.7779761
CGGGAAT48700.08.6545211
TACACGA48250.08.50857133
CCGGCAT53650.08.4355561
TAAGTCG23050.08.38160320
CGCAGAC85750.08.3799491
CGCGAAA14450.08.35616843
Produced by FastQC (version 0.11.5)