FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33373163
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG54600.012.94850814
CGCTCGT64050.011.03807315
TTACACG36800.011.0344332
CGCAGAC95150.010.7811951
CGAAGAT118050.010.439621
ACCCGAT75250.09.53537268
CCGGCAT57100.09.503941
CTCGTAC77100.09.44212917
ACTTCGC80900.09.43056611
TAAGTCG34200.09.41437420
CTCGGAT79850.09.3392671
CGCGCAT13150.09.3185941
CCGGGAT108550.09.2890381
ACTCGTG79500.09.20164622
TCGGGTC50950.09.1363832
CGCCGAT48800.09.1115441
CGGGAAT59100.09.0045941
CGCGACT12100.08.9698331
CGCGGGT26200.08.9532681
GCTCGTA85600.08.87251916
Produced by FastQC (version 0.11.5)