FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18604014
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46750.041.76383243
CGTATGC48800.039.9390444
TATGCCG49550.039.4055146
GTCACGA54750.035.9132829
GATCGGA55000.034.5154951
AGTCACG57600.033.775928
CTCGTAT56700.033.52231642
ACACGTC64000.031.09814513
CACGATC64800.030.39638731
CACGTCT69650.028.67451514
ATGCCGT68200.028.42733247
TCACGAT70450.027.81196430
ACGATCA71600.027.60600932
ACGTCTG73450.027.42582515
ATCTCGT68600.027.05300340
CACACGT75800.026.620712
TCTCGTA71450.025.97377441
TCGGAAG74600.025.6291923
CGATCAG78200.025.40842433
ATCGGAA76450.024.8284912
Produced by FastQC (version 0.11.5)