FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18604014
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG15750.017.9535483
GTATCGA17300.014.750484
CGTATCA19750.014.50224850
CCCGTAT18250.013.7969211
TACGCGA13000.013.00559755
TTACGCG12500.012.97332854
GCGTCGT24050.012.04646112
CATACGA9700.011.37770815
CGCCGTA24500.011.26797647
TATCGCG4301.0628355E-711.23225722
GATCGGA30050.011.0191711
CGCGATA2550.001538835210.8176212
GCGGTAT25050.010.7401171
ATCGAAG35900.010.3754256
ACGCGAC16850.010.23620556
GCCGTAT27700.010.21547248
TTACACG17500.010.05285432
CGCGAAT7252.3646862E-119.9908721
GTTACGC17500.09.46418353
CGGCATA13200.09.40640812
Produced by FastQC (version 0.11.5)