FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34167784
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41800.015.68110449
TCGTATG42900.015.11819346
CGTATGC48650.013.5440947
TCGCTCG47200.012.13304714
CGCTCGT49450.011.929811515
CTCGTAT56950.011.81245645
CGAAGAT114800.011.3515471
TTACACG40200.011.15627432
TATCTCG59500.011.01630542
CGACGAT24600.010.6790861
GTTCCGT58700.010.63756836
CGATCCA50950.010.5651364
CGTATCT60250.010.5356240
TCCGTAT61050.010.4540738
CGCAGAC79000.010.4137111
CGCGATT9300.010.03543951
CCGTATC64500.09.89489839
CCGGCAT50650.09.6226631
ACACGTC67450.09.61567214
GATCGGA65800.09.59822
Produced by FastQC (version 0.11.5)