FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45803948
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG50050.013.2865114
CGCGAAA18700.011.04269743
TTACACG53400.010.55229732
CGCTCGT63450.010.48053415
CGCGTAT7351.8189894E-1210.4784211
CGAAGAT166200.010.4053441
CGCGGGT22200.010.0922381
CGATCCA70000.010.0498474
CGCAGAC109250.09.8052911
CGCAAAT67400.09.7646891
CTCGGAT97350.09.7452691
CGACCGT17700.09.6913281
CGGACAT72350.09.677251
CCGGGAT97600.09.5768341
CGCGGAT31000.09.4858891
CGGAAAT86600.09.2975911
CGTATCG36500.09.2054093
CTCGTTT67650.09.107631
CTCGGGT89300.08.9772751
TATGCCG64850.08.9590683
Produced by FastQC (version 0.11.5)