FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45803948
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG52550.013.32059514
CGCGGGT23650.011.2472631
CGCTCGT66900.010.7249215
CGCCGAT69450.010.3814831
TCGGGTC74400.010.3493772
CGACCGT20350.010.31932451
CTCGGGT92100.010.2985031
CCGGGAT101500.010.0688811
TTACACG53550.010.00010332
CGACCTA26500.09.9055794
CGAAGAT167800.09.8449721
CGCAGAC111500.09.825031
CGATCCA71200.09.5364374
CCGGAAT67050.09.4480951
CGCGCCG22150.09.0066981
CCGGCAT64100.08.845481
CTCGGAT99400.08.7675321
CGCGAGT16200.08.6419041
GCTCGTA84150.08.609591516
CGACGAT31850.08.5713571
Produced by FastQC (version 0.11.5)