FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26449911
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG16550.013.340981554
GTATCGA24650.013.2948864
CGTATCG23250.012.7600073
CCCGTAT27150.011.818481
TACGCGA18700.011.80705855
GCGGTAT39200.011.61813451
CGCGTAA19800.010.9780171
CGCGGAT24550.010.9620471
CGATCCA38600.010.8137424
ATCGAAG53300.010.6793626
CCGGGAT51250.010.2328881
GCGGAAT30600.010.0349921
CCGTATC36850.010.0166312
TATCGCG5201.3512727E-79.95103622
ACGCGAC22550.09.79216456
TAACGCC29600.09.43983929
CTCGGAT56600.09.3875621
GTCGCTA19600.09.32805627
CGCCGAT45000.09.2772951
CCGGCAT51750.09.2672941
Produced by FastQC (version 0.11.5)