FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27759874
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG55000.033.80793443
TATGCCG60150.031.20447246
CGTATGC62050.030.24804944
CTCGTAT66750.027.6508942
GATCGGA63800.027.5290581
ACACGTC74550.027.4417213
ATCGGAA70050.024.8723282
ACGTCTG82800.024.58249315
CACGTCT91350.022.65932514
ATCTCGT88250.020.8750240
ATGCCGT90050.020.65183347
TCTCGTA93500.019.3342441
CGTCTGA106300.019.3091416
CACACGT108550.018.87821412
TCGGAAG100800.017.8324243
GCACACG121350.017.31337711
GCCGTCT112900.016.8386249
CCGTCTT114150.016.5934850
GTATGCC120950.015.57541245
TGCCGTC126750.015.05315748
Produced by FastQC (version 0.11.5)