FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35770368
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT64250.032.26383642
TATGCCG73200.028.8847746
ACACGTC76950.028.28809213
GATCGGA78350.026.3819641
CGTATGC83800.025.31337244
ACTTAGG86900.024.64806632
ATGCCGT86600.024.29581347
CACGTCT92350.023.72051214
TCGCTAT79150.023.27026728
CACTTAG91450.023.23314731
AAGCGAC81300.022.65503520
TCGTATG94400.022.25156443
CCGACAT90050.021.996191
CTTAGGC105750.021.00470233
CCGGTAT102800.020.9800341
CGCTATG88050.020.95730629
TTAGGCA107600.020.83603934
GTCGCTA88750.020.83087327
CACACGT105700.020.75730912
GAACGCT92400.019.85883335
Produced by FastQC (version 0.11.5)