FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004859277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83321601
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG357400.030.4411145
TATGCCG383300.028.49033248
CGTATGC389800.027.97320246
CTCGTAT385200.027.60479444
TATCTCG410050.026.48706241
TCTCGTA424050.024.89797643
AGATCGG376650.023.627681
GTCACGA459950.023.60643629
ATCTCGT459050.023.05148542
ATGCCGT478700.022.97859849
AGTCACG476400.022.8903728
GATCGGA499700.022.8187371
CACGAGT459550.022.6369231
ACGAGTG469200.022.59497632
ACACGTC499050.022.2426213
ATCGGAA527950.021.644982
ACGTCTG530950.021.20465315
TCACGAG539700.020.44916230
CACGTCT546800.020.44820814
GCCGTCT540000.020.3703351
Produced by FastQC (version 0.11.5)