FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19470482
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG67150.034.9220281
GATCGGA76200.033.8852421
ATCGGAA83300.031.0972462
TCGTATG93500.024.59733246
CGTATGC104600.022.23062747
TATGCCG104300.022.18990149
TCGGAAG119050.021.5909063
CTCGTAT100300.021.1882245
AGTCACG112900.020.37728329
GTCACGT116550.019.56860530
TGAAACG115350.019.43063436
ACGATCT105850.019.25445640
ACACGTC119300.019.08863814
AACGATC108500.018.81438439
ATGCCGT123450.018.74772650
ACGTCTG123900.018.43283316
GCCGTCT124400.018.22709352
GAAACGA124300.018.11065137
ATCTCGT114300.017.91696443
AAACGAT124550.017.75790638
Produced by FastQC (version 0.11.5)