FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19470482
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA29750.042.1132515
CGGCATA32000.037.7780212
GCATACG32300.037.5202314
CGTATCA61800.036.5554350
ATACGAG38550.030.81721716
GTCGCCG77300.029.22497645
CGCCGTA78150.028.90688947
GCCGTAT78750.028.81560948
GCGTCGT81900.028.2703812
AGATCGG84400.028.0180231
TCGCCGT80600.027.01228546
TGGTCGC81950.026.48415443
GATCGGA91900.026.0280342
CCGTATC87650.025.9679349
GTATCAT90200.025.49973951
AGCGTCG92250.025.39133811
GGTCGCC93500.023.97903444
ATCGGAA100400.023.8585763
AGAGCGT98800.023.7426269
TCTCGGT99650.023.7308337
Produced by FastQC (version 0.11.5)