FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25730666
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46800.025.22548943
TATGCCG48500.024.20013846
CGTATGC51500.022.72402244
CTCGTAT57000.020.8316842
ACACGTC62800.019.61582813
GATCGGA59350.018.3860661
ACGTCTG71850.017.3357515
TCTCGTA67800.017.16006541
ACTTAGG74400.015.959475532
CACGTCT77700.015.89842814
ATCGGAA71600.015.2674182
CACTTAG78450.015.09212331
TTAGGCA83050.015.08007734
ATGCCGT84000.014.82817147
ATCTCGT79200.014.68982740
CTTAGGC84150.014.59830533
CACACGT91100.013.63490212
TCACTTA92950.013.03232430
TAGGCAT92800.012.90569335
CGTCTGA96100.012.81869316
Produced by FastQC (version 0.11.5)