FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25730666
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA17550.018.2806915
CGTATCA28100.014.61153449
GCATACG24300.013.486649514
CGGCATA25150.012.34620612
GATCGGA51750.012.1431761
CGAAGAT81250.011.5164121
GCGTCGT42400.011.31039911
CGCCGTA36700.010.80696446
TCGCTCG37800.010.67777614
CCGCAAT30000.010.3583961
CCGGGAT64200.010.3261271
ATACGAG27400.010.32404916
GCCGTAT41200.010.0493447
CGCGATT9550.09.7618391
CGCCAAT48300.09.7221661
TAAGCGA15700.09.66551346
CGCAGAC80850.09.523491
CGGGAAT47050.09.39337351
ATCGGAA65450.09.3253382
ATTAGCG9350.09.2181926
Produced by FastQC (version 0.11.5)