FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28800692
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA42100.040.3124715
GCATACG52050.037.2457314
CGGCATA49000.037.17511712
CGTATCA46700.036.34676450
GCCGTAT51900.033.1704648
CGCCGTA53200.032.230247
ATACGAG47200.031.79057316
CCGTATC61600.027.9471949
GCGTCGT66750.026.30758912
TGGTCGC65450.024.61615643
GTCGCCG69900.023.7399745
AGACGGC106550.021.693859
ACGGCAT84200.021.6749511
AAGACGG105650.021.6501838
GATCGGA88200.021.5937862
GGTCGCC78450.021.37247544
TCGTGTA85200.020.76988815
GTATCAT85100.020.55376651
GTAGATC85850.020.5350232
GTCGTGT87750.020.20584114
Produced by FastQC (version 0.11.5)