FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37565940
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA25650.014.25061
CGTATCG33500.014.2068233
GCGGTAT57550.012.8228081
TTACGCG18400.012.56466254
CCCGTAT42500.012.4141541
GTATCGA38200.012.1878084
CGATCCA58400.011.3972394
TACGCGA21050.011.143332555
TATCGCG9900.010.45889822
CGGTATA25100.010.4424272
CCGTATC58100.010.3877972
TTACACG42900.010.29330832
TCGGGTC78600.010.1356352
CGACCTA20450.09.7811494
GCGGAAT43500.09.7505811
CGCCGAT69150.09.6744041
CGGGGTT99900.09.6651191
ATCGAAG72900.09.6091746
GCGTAAC38500.09.4952562
TCGTTCG40050.09.2207728
Produced by FastQC (version 0.11.5)