FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29008164
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26150.017.6779243
TATGCCG31800.014.42857146
TCGCTCG40400.013.32127514
CGCGAAA13400.013.1299643
CGTATGC35400.012.86378344
TATCGCG6650.011.9323920
CGAAGAT94500.011.6273991
TATCTCG38950.011.51412639
AGTCACG40550.011.4875828
CTCGTAT47150.011.12141242
ACACGTC42600.011.01366413
CGCGTAT4753.5030098E-810.9115111
CGCGATT9650.010.7419031
CCGGGAT48150.010.1901221
TTACACG32700.010.02243332
CGCGAAC8700.09.9290581
CGCGCAT12050.09.7494291
GATCGGA41100.09.6681491
GTATCGA30150.09.3828564
ACTCGTG57950.09.34789822
Produced by FastQC (version 0.11.5)