FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29008164
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG41100.013.51461314
TTACACG35300.012.996984532
CGAAGAT98650.012.567161
CCGGGAT52950.011.5437551
ACTCGTG55750.010.27177122
CGCGATT11850.010.1997491
TATCGCG7851.8189894E-1210.11233520
CCCGATA56650.09.68170169
CGCGCAT13000.09.5631061
CGCTCGT58850.09.56016115
CGACCTA26450.09.5227354
CGCAGAC91500.09.5108471
CGACTAA27100.09.03755628
TATATCG14250.08.95677835
GTATCGA29400.08.91934
CTCGTAC64400.08.89705517
CGGAGAT82550.08.8686721
TACACGA55200.08.81140133
CGCCAAT57250.08.806781
TTCGCTC67400.08.75297613
Produced by FastQC (version 0.11.5)