FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30454490
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG57000.038.1877543
TATGCCG62150.034.80128546
CGTATGC63400.034.44165844
GATCGGA72300.031.462281
CTCGTAT66250.030.66885642
ACACGTC76450.030.5533313
CACGTCT94400.024.99950614
ACGTCTG97200.024.17290715
ATGCCGT91400.024.11697447
ATCGGAA97300.023.2954622
TCTCGTA89600.023.0229741
CACACGT106150.022.10093112
ATCTCGT96800.021.31051840
TCGGAAG112450.020.5219083
GCACACG120050.020.45779811
AATCTCG106100.020.4179339
GCCGTCT111900.019.51373349
GACCAAT121800.018.66927735
ACCAATC117650.018.64798536
TGCCGTC122300.018.41852648
Produced by FastQC (version 0.11.5)