FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25650535
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG21500.013.43607554
TACGCGA23200.012.45046755
GCGGTAT40650.011.1527171
ATCGGTA22350.010.28496818
ATCGAAG45600.010.2304556
CCAATCG24200.09.90271715
GTTACGC29750.09.82240153
ACGCGAC32050.09.31875156
ATCGAAC40750.09.1100826
CCGGAAT56250.08.8773481
GTATCGA21750.08.760544
CGCCGAT37550.08.7488751
CGTATCA20050.08.68082250
TATCGCG6457.518611E-88.65892722
CGCGGAT20250.08.5983081
CATACGA13750.08.595016515
GTTACGA15750.08.5519564
CGACCGT30500.08.5057132
TCGACCG29800.08.48518131
CCGACAT46100.08.4802411
Produced by FastQC (version 0.11.5)