FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37901571
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG48000.013.41300614
CGTATCG30450.011.0312243
CGCTCGT60900.010.85910515
CGCGTAT6552.7284841E-1110.6964541
TCGTATG61100.010.54044845
CGAAGAT127150.010.3591141
TATGCCG64450.010.31860948
CGCGAAC9650.010.1644021
CTCGGTT46650.010.1375561
CGCGATT13750.09.9360331
CGTATGC67250.09.88903946
GTATCGA40800.09.7765254
CCCGTAT44600.09.6609841
CGCGCAT14550.09.6304841
CGTTCGC21850.09.6083616
CGCAGAC114400.09.4620141
CGGTAAT25900.09.3325531
CGCGAAT16550.09.3133471
CGCCAAT69250.09.2066741
TTACACG43900.09.09225932
Produced by FastQC (version 0.11.5)