FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28880042
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65850.026.0856531
ACACGTC79300.023.221213
ATCGGAA77150.022.157452
GTCACGA50000.020.54326429
ACGTCTG91200.020.37906315
AGTCACG53500.020.09994528
CACGTCT97700.019.12905914
TCGTATG66200.018.8369343
TATGCCG68550.018.3456446
CGTATGC69500.017.94487444
ACGATCA60400.017.00592232
CACACGT110750.016.7821112
GCACACG115300.016.68598211
CTCGTAT75400.016.58408542
CACGATC61500.016.4224931
CGTCTGA114200.016.30407516
TCGGAAG108350.016.2025283
TATCGCG6150.016.20053920
CGATCAG64350.016.0152433
TCACGAT72650.014.37489530
Produced by FastQC (version 0.11.5)