FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28880042
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA29500.021.40374850
CGCCGTA30950.021.07009347
GCCGTAT33100.019.18028648
GCGTCGT36050.017.79785212
GATCGGA46550.015.5708822
CCGTATC46400.014.351541549
TCGCTCG36100.013.28198314
ATCGGAA56250.013.1314393
CTCGGGT67050.012.560151
GTCGCCG54050.012.25679245
TATCGCG7450.012.0384420
AGAGCGT56000.012.0116729
TGGTCGC51850.011.97700943
TCGGGTC61550.011.6640362
TCGTGTA56450.011.48822815
GGTCGCC57300.011.37992344
CTCGGAT72600.011.2196461
CGTGTAG62750.011.10430816
AGCGTCG60400.010.85103811
CGAAGAT94200.010.8453761
Produced by FastQC (version 0.11.5)