FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22442265
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4702.404704E-911.7574641
CGAAGAT69550.011.322161
TCGCTCG28250.010.74035714
TACGTAG20850.010.251429
CTCGAAT39200.09.8678721
GTACGTA22600.09.76278
CGCAAAT26450.09.662681
CGGGAAT36100.09.4715181
CTCGGTT28500.09.4523831
CGCCAAT35850.09.2485491
CGCGGAT22100.09.2204331
CGCCGAT35350.09.0862581
CGCAGAC66850.08.989581
CGGAAAT41200.08.8858761
CGGCAAT34100.08.7103511
CCGCAAT40450.08.7090991
CGATCCA35000.08.6830874
CGAAAAC27850.08.556881
CGGACAT40800.08.5497371
CCGGGAT63550.08.4237941
Produced by FastQC (version 0.11.5)