FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22442265
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG4651.0913936E-1113.35386320
CGCGAAT7750.011.1179241
CGCGTAT4107.0561873E-710.9281061
CGACCGT12200.010.7351761
CGAAGAT68200.010.6125641
CGGAAAT40400.09.9813641
TCGCTCG32150.09.86908714
CGCGCAT9500.09.7954761
GTATCGA17500.09.4544464
CGCGATT6302.3605025E-89.3002321
TATATCG11150.09.28220635
CGCCGAT35650.09.281051
TACGTAG21700.09.2104959
CCGGAAT37550.09.1785791
CGCCAAT36450.09.1719061
CGGAGAT57150.09.1666951
CGAACAT49000.09.0735871
CCGGCAT48850.09.0308951
GTACGTA23000.08.9904568
CTCGGAT55600.08.9883211
Produced by FastQC (version 0.11.5)