FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21589200
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA21650.033.29945415
CGGCATA27650.030.6916312
GCATACG28200.030.21585514
TATCGCG6950.025.81086322
ATACGAG30250.022.57821316
TACGAGA37850.015.7669317
ACGGCAT55650.015.37331511
AGACGGC71850.015.3639969
GCCGTAT23900.015.156486548
TTACACG29300.014.95556232
CTCGTTT37300.014.7979031
CGTATCA24200.014.53977750
ATACGCG3101.8235369E-814.46543216
CGAGATT37200.014.37343919
CGACTTA16050.013.75800567
AAGACGG71100.013.6824398
CGCCGTA27050.013.3918147
CGAAGAT83100.013.3257931
ACGAGAT42150.012.9310118
CTCGGGT48900.012.7690531
Produced by FastQC (version 0.11.5)